1.1 This laboratory test method is designed to measure efficacy of ready-to-use dry bait formulations prepared with multiple-dose (anticoagulant) lethal rodenticides for the control of commensal rodents, including the Norway rat (Rattus norvegicus), roof rat (R. rattus), and the house mouse (Mus musculus), but may also be applicable to some other species of rodents having behavioral patterns, physiological, and feeding preferences similar to those mentioned.

1.2 This test method is for the use of those wishing to develop efficacy data.

1.3 This test method may also prove valuable to manufacturers, or formulators, or both and others by providing a test procedure for monitoring product quality.

1.4 This method, within limits, will enable users of large quantities of commercial rodenticides to evaluate the efficacy of specific lots.and either evaluate or compare the efficacy of different formulations, or different toxicants, or any combination thereof.

1.5 The details of this test method are fairly specific in order to reduce many variables and make it possible to replicate or duplicate tests with reasonable accuracy.

1.6 This test method is not intended to be so restrictive that it will inhibit incentives towards the development of safer or more effective compounds or innovative approaches to bait formulation.

1.6.1 When justified by sound biological data or logical conclusions based on sound data, reasonable variations to some items in the test protocol may be made, provided such change does not give an unrealistic efficacy advantage to the test bait.

1.7 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.

1.8 This test method represents the combined, several-years efforts, of more than 70 scientists, users, and laboratory specialists from several countries and has not been superseded by better documentation (1995); therefore, it is the view of the committee that it be continued for the reference benefit of any new concerns in the United States or abroad.

1.9 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.