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ASTM D5244

1992 Edition, May 15, 1992

Complete Document

Standard Practice for Recovery of Enteroviruses from Waters

Includes all amendments and changes through Reapproval Notice , 2004


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Product Details:

  • Revision: 1992 Edition, May 15, 1992
  • Published Date: January 2004
  • Status: Not Active, See comments below
  • Document Language: English
  • Published By: ASTM International (ASTM)
  • Page Count: 3
  • ANSI Approved: Yes
  • DoD Adopted: No

Description / Abstract:

This practice covers a uniform procedure for the concentration of viruses from collected samples.

This practice describes a virus adsorption-elution cartridge filter procedure for recovering viruses from drinking water. Volumes of 400 L or more are processed for samples of drinking water quality.

The principles of this practice are also applicable to sewages, effluents, and surface waters without technical modifications.

Although specifically designed for recovery of human enteroviruses, this practice also may be applied to some other human enteric viruses, that have to be determined by specific testing.

The consistency of this practice was determined from method evaluation studies with poliovirus-seeded drinking water samples.

The values stated in SI units are to be regarded as the standard.

This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Only adequately trained personnel should be allowed to perform these procedures and should use safety precautions recommended by the U.S. Public Health Service Center for Disease Control for work with potentially hazardous biological organisms.(Footnote 2)

Footnote 2 - Biological Safety in Microbiological and Biomedical Laboratories, Richardson, J. H., and Barkley, W. E., Eds., U.S. Dept. of Health and Human Services, Public Health Service, Centers for Disease Control and National Institutes of Health, HHS Publication No. (NIH) 88-8395, 2nd Ed, May 1988.