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ASTM E1383

Revision 94A, July 15, 1994

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Standard Guide for Conducting Sediment Toxicity Tests with Freshwater Invertebrates

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Superseded By: ASTM E1706

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Product Details:

  • Revision: Revision 94A, July 15, 1994
  • Published Date: July 15, 1994
  • Status: Superseded By:
  • Superseded By: ASTM E1706
  • Document Language: English
  • Published By: ASTM International (ASTM)
  • Page Count: 30
  • ANSI Approved: No
  • DoD Adopted: No

Description / Abstract:

1. Scope

1.1 This guide covers procedures for obtaining laboratory data to evaluate adverse effects of contaminants associated with whole sediment on freshwater organisms. The methods are designed to assess the toxic effects on invertebrate survival, growth, or reproduction, from short- (for example, 10 days) or long-term tests, in static or flow-through water systems. Sediments to be tested may be collected from field sites or spiked with known compounds in the laboratory. Test procedures are described for (1) Hyalella azteca, (2) Chironomus tentans, (3) Chironomus riparius, (4) Daphnia sp. and Ceriodaphnia sp., (5) Hexagenia spp., (6) Tubifex tubifex. Methods described in this guide should also be useful for conducting sediment toxicity tests with other aquatic species, although modifications may be necessary.

1.2 Modification of these procedures might be justified by special needs. Results of tests conducted using unusual procedures are not likely to be comparable to results using this guide. Comparison of results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting sediment toxicity tests with freshwater organisms.

1.3 The results from field-collected sediments used in toxicity tests to determine a spatial or temporal distribution of sediment toxicity may be reported in terms of the biological effects on survival, growth, or reproduction (see Section 16, Calculation). In addition, these procedures are applicable to most sediments or chemicals added to sediment. Materials either adhering to sediment particles or dissolved in interstitial water can be tested. With appropriate modifications these procedures can be used to conduct sediment toxicity tests when factors such as temperature, dissolved oxygen, pH, and sediment characteristics (for example, particle size, organic carbon content, and total solids) are of interest, or when there is a need to test such materials such as sewage sludge, oils, and particulate matter. These methods might also be useful for conducting bioaccumulation tests.

1.4 Results of toxicity tests with test materials experimentally added to sediments may be reported in terms of an LC50 (median lethal concentration) and sometimes an EC50 (median effect concentration). Results of tests may be reported in terms of an NOEC (no observed effect concentration) and LOEC (lowest observed effect concentration).

1.5 This guide is arranged as follows:


A1. Hyalella azteca (Amphipoda)

A2. Chironomus tentans (Diptera)

A3. Chironomus riparius (Diptera)

A4. Daphnia sp. and Ceriodaphnia sp.

A5. Hexagenia spp.

A6. Tubifex tubifex (Müller 1174)

A7. Diporeia spp.

1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific hazard statements are given in Section 8.