1991 Edition, September 15, 1991
Standard Guide for Acute Toxicity Test with the Rotifer Brachionus
Includes all amendments and changes through Reapproval Notice , 2012
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Description / Abstract:
This guide describes procedures for obtaining laboratory data
concerning the acute toxicity of chemicals and aqueous effluents
released into fresh, estuarine, or marine waters. Acute toxicity is
measured by exposing Brachionus newly hatched from cysts
to a series of toxicant concentrations under controlled conditions.
This guide describes a test for using B.
calyciflorus, a fresh water rotifer, and the Appendix
describes modifications of this test for estuarine and marine
waters using B. plicatilis. These procedures lead to an
estimation of acute toxicity, including the concentration expected
to kill 50 % of the test rotifers (LC50) in 24 h. Procedures not
specifically stated in this guide should be conducted in accordance
with Guide E729 and Guide E1192.