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ASTM E1470

1992 Edition, March 15, 1992

Complete Document

Standard Test Method for Characterization of Proteins by Electrophoretic Mobility

Includes all amendments and changes through Reapproval Notice , 2006

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Product Details:

  • Revision: 1992 Edition, March 15, 1992
  • Published Date: January 2006
  • Status: Not Active, See comments below
  • Document Language: English
  • Published By: ASTM International (ASTM)
  • Page Count: 3
  • ANSI Approved: No
  • DoD Adopted: No

Description / Abstract:

This test method describes a procedure for determining the electrophoretic mobility of proteins of molecular weight greater than 10 000 Daltons.

This test method uses automatic Electrophoretic Light Scattering (ELS) principles to determine the electrophoretic mobility.

The instrument2 simultaneously measures the Doppler shifts of scattered light at four different angles to determine the electrophoretic mobility distribution of protein particles. The mobility is expressed as µm-cm/V-s (micron-centimeter/volt-second).

This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

2 The sole source of supply of the instrument (Coulter Delsa 440, trademark) known to the committee at this time is Coulter Corporation, 601 W. Coulter Way, Hialeah, FL 33010. If you are aware of alternative suppliers, please provide this information to ASTM International Headquarters. Your comments will receive careful consideration at a meeting of the responsible technical committee,1 which you may attend.

1 This test method is under the jurisdiction of ASTM Committee E48 on Biotechnology and is the direct responsibility of Subcommittee E48.03 on Unit Processes and Validation.