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Revision A, June 1, 2006

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Methods for Antimicrobial Disk Susceptibility Testing of Bacteria Isolated From Aquatic Animals; Approved Guideline

Includes all amendments and changes through Reaffirmation Notice , April 2016

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VOL 26; NO 23
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Description / Abstract:

This CLSI guideline provides veterinary and aquatic animal disease diagnostic laboratories with currently recommended antimicrobial disk diffusion susceptibility testing methods for bacteria isolated from aquatic animals–primarily Group 1 organisms–including criteria for quality control testing with two quality control strains.

The document also provides appendixes and tables outlining recommended disk concentrations, antimicrobial agents used in global aquaculture, methods for preparing stock solutions and dilutions of antimicrobial agents, and a list of bacteria pathogenic to fish.

Interpretive criteria are not addressed in this guideline. Such criteria must be established using pharmacokinetic and pharmacodynamic data, in vitro susceptibility testing data, and clinical efficacy data. Developing interpretive criteria was beyond the scope of this document. As more aquatic animal-specific information becomes available, this document, and CLSI document M49—Methods for Broth Dilution Susceptibility Testing of Bacteria Isolated From Aquatic Animals, will be revised to incorporate those data.