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DS/CEN ISO/TS 15216-1

2013 Edition, June 18, 2013

Complete Document

Microbiology of food and animal feed – Horizontal method for determination of hepatitis A virus and norovirus in food using real-time RT-PCR – Part 1: Method for quantification

Includes all amendments and changes through Change/Amendment , June 18, 2013


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Product Details:

  • Revision: 2013 Edition, June 18, 2013
  • Published Date: June 18, 2013
  • Status: Not Active, See comments below
  • Document Language: English
  • Published By: Dansk Standardiseringsrad (DS)
  • Page Count: 46
  • ANSI Approved: No
  • DoD Adopted: No

Description / Abstract:

ISO/TS 15216-1:2013 describes a method for quantification of levels of HAV and NoV genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR. This approach is also relevant for detection of the target viruses on fomites, or of other human viruses in foodstuffs, on food surfaces or on fomites following appropriate validation and using target-specific primer and probe sets.