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ISO 11815|IDF 157:2007 describes a method for the determination of the total milk-clotting activity of bovine rennet containing only chymosin and bovine pepsin as the active coagulating enzymes on a standard milk substrate at pH 6,5.

To produce accurate results with this method, check test samples of unknown origin for the absence of main milk-clotting enzymes of non-bovine origin by using an appropriate method.

ISO 11815|IDF 157:2007 can also be applied to determine the total milk-clotting activity of fermentation-produced chymosin.