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ISO 17099

1st Edition, November 15, 2014

Complete Document

Radiological protection - Performance criteria for laboratories using the cytokinesis block micronucleus (CBMN) assay in peripheral blood lymphocytes for biological dosimetry

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Description / Abstract:

This International Standard addresses the following:

a) confidentiality of personal information for the customer and the laboratory;

b) laboratory safety requirements;

c) radiation sources, dose rates, and ranges used for establishing the calibration reference dose-effect curves allowing the dose estimation from CBMN assay yields and the minimum resolvable dose;

d) performance of blood collection, culturing, harvesting, and sample preparation for CBMN assay scoring;

e) scoring criteria;

f) conversion of micronucleus frequency in binucleated cells into an estimate of absorbed dose;

g) reporting of results;

h) quality assurance and quality control;

i) informative annexes containing examples of a questionnaire, instructions for customers, a microscope scoring data sheet, a sample report and advice on strengths and limitations of current automated systems for automated micronucleus scoring.
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