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ISO DIS 17601

2013 Edition, September 26, 2013

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SOIL QUALITY - ESTIMATION OF ABUNDANCE OF SELECTED MICROBIAL GENE SEQUENCES BY QUANTITATIVE REALTIME PCR FROM DNA DIRECTLY EXTRACTED FROM SOIL



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Superseded By: ISO 17601

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NOW A PUBLISHED STD * SEE ISO 17601
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Description / Abstract:

This International Standard specifies the crucial steps of a quantitative real-time polymerase chain reaction (qPCR) method to measure the abundance of selected microbial gene sequences from soil DNA extract which provides an estimation of selected microbial groups.

It is noteworthy that the number of genes is not necessarily directly linked to the number of organisms that are measured. For example, the number of ribosomal operon is ranging from one copy to 20 copies in different bacterial phyla. Therefore the number of 16S rRNA sequences quantified from soil DNA extracts does not give an exact estimate of the number of soil bacteria. Furthermore the number of sequences is not necessarily linked to living microorganisms and can comprise sequences amplified from dead microorganisms.
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