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ISO FDIS 20130

2018 Edition, March 22, 2018

Complete Document

SOIL QUALITY - MEASUREMENT OF ENZYME ACTIVITY PATTERNS IN SOIL SAMPLES USING COLORIMETRIC SUBSTRATES IN MICRO-WELL PLATES



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Description / Abstract:

This document specifies a method for the measurement of several hydrolase activities (arylamidase, arylsulfatase, β-galactosidase, α-glucosidase, β-glucosidase, N-acetyl-glucosaminidase, acid, alkaline and global phosphatases, urease) simultaneously (or not) in soil samples, using colorimetric substrates. Enzyme activities of soil vary seasonally and depend on soil chemical, physical and biological characteristics. This method can be applied either to detect harmful effects on soil enzyme activities derived from toxic substances or other anthropogenic agents in contaminated soils against a control soil, or to test chemicals.
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