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VDI 4330 BLATT 11

2015 Edition, September 1, 2015

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Monitoring the effects of genetically modified organisms (GMO) - Immunochemical detection of insecticidal Bt proteins from genetically modified crops in soil samples and plant residues

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Product Details:

  • Revision: 2015 Edition, September 1, 2015
  • Published Date: September 2015
  • Status: Active, Most Current
  • Document Language: German, English
  • Published By: Deutsches Institut fur Normung E.V. (DIN)
  • Page Count: 12
  • ANSI Approved: No
  • DoD Adopted: No

Description / Abstract:

This standard describes the basic procedure and the requirements of methods based on immunochemical detection for the extraction and qualitative and quantitative determination of Bt proteins from genetically modified plant residues found on the field after harvesting, and soil samples which have come into contact with these plants. The specificity of the methods for the Bt proteins depends on the specificity of the immunochemical agents (antibodies) used. In some circumstances, immunoreactive degradation products may also be detected, which prevents the immediate detection of the biological, e.g. insecticidal activity of these proteins. Depending on the specificity of the immunochemical detection concerned, a priori cross reactions with naturally occurring Bt proteins, such as those produced by Bacillus thuringiensis strains present in soil, cannot be ruled out.

The standard VDI 4330 Part 1 presents the necessary fundamentals for the understanding of this standard.